normal human bronchial epithelial cells hbe  (ATCC)

Journal: Cancer Cell International

Article Title: Circulating extracellular vesicle PTPRO methylation: an exploratory biomarker for minimally invasive diagnosis of early-stage lung adenocarcinoma

doi: 10.1186/s12935-025-04127-9

Figure Lengend Snippet: Methylation analysis of PTPRO in LUAD cell lines and LUAD cells derived sEVs. (A) The mRNA expression levels of PTPRO were determined by RT-qPCR in the normal lung epithelial cell line HBE and LUAD cell lines A549, NCI-H460, and PC-9. (B-C) PTPRO methylation status in HBE and the LUAD cell lines A549, NCI-H460, and PC-9 was assessed using MSP and q-MSP assays. (D) Representative TEM images of sEVs purified from the conditioned media of HBE and LUAD cell lines (A549, NCI-H460, and PC-9). Scale bar: 100 nm. (E) The concentration and size distribution of sEVs from HBE and LUAD cell lines (A549, NCI-H460, and PC-9) were evaluated using NTA. (F) Expression of sEV markers (TSG101, Alix, CD63, and Calnexin) and PTPRO in sEVs purified from HBE and LUAD cell lines (A549, NCI-H460, and PC-9) was detected by immunoblotting. (G) The mRNA expression levels of PTPRO in sEVs purified from the conditioned media of HBE and LUAD cell lines (A549, NCI-H460, and PC-9) were measured by RT-qPCR. (H-I) MSP and q-MSP analyses of purified sEVs from HBE and LUAD cell lines (A549, NCI-H460, and PC-9). H 2 O was used as a negative control. Error bars indicate SEM. *** P < 0.001 by one-way ANOVA with Dunnett's multiple comparisons test.

Article Snippet: Human LUAD cell lines (A549, PC-9, and NCI-H460) and normal human bronchial epithelial cells (HBE) were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Methylation, Derivative Assay, Expressing, Quantitative RT-PCR, Purification, Concentration Assay, Western Blot, Negative Control

Journal: Clinics (Sao Paulo, Brazil)

Article Title: Inhibition of NSUN3 suppresses immune escape in non-small cell lung cancer through stabilizing PD-L1 in a 5-methyladenosine dependent way.

doi: 10.1016/j.clinsp.2025.100662

Figure Lengend Snippet: Fig. 1. NSUN3 levels were upregulated in NSCLC. (A) Quantitative histogram of NSUN3 mRNA levels in NSCLC tissues and normal lung tissues from qPCR analysis (n = 22). (B) Representative protein images which represent the NSUN3 protein levels in six paired clinical samples with NSCLC and adjacent tissues analyzed by western blot (n = 3). (C) The ROC curve of NSUN3 in NSCLC. (D) Relative mRNA expression of NSUN3 in human normal bronchial epithelial cell line HBE and NSCLC cell lines (A549 and PC9) from qPCR analysis (n = 3). (E) Protein levels of NSUN3 in HBE, A549 and PC9 cells are determined by western blot (n = 3).

Article Snippet: Human NSCLC cell lines (A549 and PC9) obtained from Beyotime Company and human normal bronchial epithelial cell line HBE obtained from ATCC were cultured in DMEM supplemented with 10 % fetal bovine serum (FBS; Gibco) and 1 % penicillin/streptomycin (Beyotime).

Techniques: Western Blot, Expressing